Serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain
The invention relates to the field of cells and biological products, in particular to a serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain. In the method provided by the invention, the Newcastle disease virus strain is a Newcastle disease gene VII type vi...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | WANG XIN ZHANG JINJUN ZHOU MINGGUANG JIN JIANYUN CHENG TAILANG XU GAOYUAN CHEN ZHANGBIAO ZHANG HONGBIN |
| description | The invention relates to the field of cells and biological products, in particular to a serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain. In the method provided by the invention, the Newcastle disease virus strain is a Newcastle disease gene VII type virulent strain attenuated by F gene locus mutation. According to the method disclosed by the invention, a serum-free full-suspension cell culture is adopted, so that the method is not influenced by insufficient hatching eggs supply or uncontrollable factors; the method disclosed by the invention has the advantages of easy operation, large virus preparation amount and high virus content, and overcomes the defect that the supply of vaccines on the market is influenced by vaccine production stagnation caused by influence on virus reproduction due to insufficient supply of hatching eggs in virus chick embryo culture.
本发明涉及细胞和生物制品领域，具体涉及一种新城疫VII型弱毒株无血清全悬浮细胞培养的方法。在本发明所述方法中，所用新城疫病毒的毒株为新城疫基因VII型强毒经F基因位点突变而致弱的毒株。本发明所述方法，采用无血 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN112961837A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN112961837A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN112961837A3</originalsourceid><addsrcrecordid>eNqNyr0KwjAUBtAuDqK-w_UBOtSCP6MUxS5dFNcS0i8YuE1C7g3i27v4AE5nOctqvCOXuXYZIFeYaymSEMTHQBbMZAtryaAZ-ooTuZhpwNsaUQZNXmAE9Ox70k8CGVWEYhQTiWbjw7paOMOCzc9Vtb1eHt2tRoojJBmLAB27oWl2p31zbA_n9p_zBZhiPR4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain</title><source>esp@cenet</source><creator>WANG XIN ; ZHANG JINJUN ; ZHOU MINGGUANG ; JIN JIANYUN ; CHENG TAILANG ; XU GAOYUAN ; CHEN ZHANGBIAO ; ZHANG HONGBIN</creator><creatorcontrib>WANG XIN ; ZHANG JINJUN ; ZHOU MINGGUANG ; JIN JIANYUN ; CHENG TAILANG ; XU GAOYUAN ; CHEN ZHANGBIAO ; ZHANG HONGBIN</creatorcontrib><description>The invention relates to the field of cells and biological products, in particular to a serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain. In the method provided by the invention, the Newcastle disease virus strain is a Newcastle disease gene VII type virulent strain attenuated by F gene locus mutation. According to the method disclosed by the invention, a serum-free full-suspension cell culture is adopted, so that the method is not influenced by insufficient hatching eggs supply or uncontrollable factors; the method disclosed by the invention has the advantages of easy operation, large virus preparation amount and high virus content, and overcomes the defect that the supply of vaccines on the market is influenced by vaccine production stagnation caused by influence on virus reproduction due to insufficient supply of hatching eggs in virus chick embryo culture.
本发明涉及细胞和生物制品领域，具体涉及一种新城疫VII型弱毒株无血清全悬浮细胞培养的方法。在本发明所述方法中，所用新城疫病毒的毒株为新城疫基因VII型强毒经F基因位点突变而致弱的毒株。本发明所述方法，采用无血</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210615&DB=EPODOC&CC=CN&NR=112961837A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210615&DB=EPODOC&CC=CN&NR=112961837A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG XIN</creatorcontrib><creatorcontrib>ZHANG JINJUN</creatorcontrib><creatorcontrib>ZHOU MINGGUANG</creatorcontrib><creatorcontrib>JIN JIANYUN</creatorcontrib><creatorcontrib>CHENG TAILANG</creatorcontrib><creatorcontrib>XU GAOYUAN</creatorcontrib><creatorcontrib>CHEN ZHANGBIAO</creatorcontrib><creatorcontrib>ZHANG HONGBIN</creatorcontrib><title>Serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain</title><description>The invention relates to the field of cells and biological products, in particular to a serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain. In the method provided by the invention, the Newcastle disease virus strain is a Newcastle disease gene VII type virulent strain attenuated by F gene locus mutation. According to the method disclosed by the invention, a serum-free full-suspension cell culture is adopted, so that the method is not influenced by insufficient hatching eggs supply or uncontrollable factors; the method disclosed by the invention has the advantages of easy operation, large virus preparation amount and high virus content, and overcomes the defect that the supply of vaccines on the market is influenced by vaccine production stagnation caused by influence on virus reproduction due to insufficient supply of hatching eggs in virus chick embryo culture.
本发明涉及细胞和生物制品领域，具体涉及一种新城疫VII型弱毒株无血清全悬浮细胞培养的方法。在本发明所述方法中，所用新城疫病毒的毒株为新城疫基因VII型强毒经F基因位点突变而致弱的毒株。本发明所述方法，采用无血</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyr0KwjAUBtAuDqK-w_UBOtSCP6MUxS5dFNcS0i8YuE1C7g3i27v4AE5nOctqvCOXuXYZIFeYaymSEMTHQBbMZAtryaAZ-ooTuZhpwNsaUQZNXmAE9Ox70k8CGVWEYhQTiWbjw7paOMOCzc9Vtb1eHt2tRoojJBmLAB27oWl2p31zbA_n9p_zBZhiPR4</recordid><startdate>20210615</startdate><enddate>20210615</enddate><creator>WANG XIN</creator><creator>ZHANG JINJUN</creator><creator>ZHOU MINGGUANG</creator><creator>JIN JIANYUN</creator><creator>CHENG TAILANG</creator><creator>XU GAOYUAN</creator><creator>CHEN ZHANGBIAO</creator><creator>ZHANG HONGBIN</creator><scope>EVB</scope></search><sort><creationdate>20210615</creationdate><title>Serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain</title><author>WANG XIN ; ZHANG JINJUN ; ZHOU MINGGUANG ; JIN JIANYUN ; CHENG TAILANG ; XU GAOYUAN ; CHEN ZHANGBIAO ; ZHANG HONGBIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112961837A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG XIN</creatorcontrib><creatorcontrib>ZHANG JINJUN</creatorcontrib><creatorcontrib>ZHOU MINGGUANG</creatorcontrib><creatorcontrib>JIN JIANYUN</creatorcontrib><creatorcontrib>CHENG TAILANG</creatorcontrib><creatorcontrib>XU GAOYUAN</creatorcontrib><creatorcontrib>CHEN ZHANGBIAO</creatorcontrib><creatorcontrib>ZHANG HONGBIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG XIN</au><au>ZHANG JINJUN</au><au>ZHOU MINGGUANG</au><au>JIN JIANYUN</au><au>CHENG TAILANG</au><au>XU GAOYUAN</au><au>CHEN ZHANGBIAO</au><au>ZHANG HONGBIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain</title><date>2021-06-15</date><risdate>2021</risdate><abstract>The invention relates to the field of cells and biological products, in particular to a serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain. In the method provided by the invention, the Newcastle disease virus strain is a Newcastle disease gene VII type virulent strain attenuated by F gene locus mutation. According to the method disclosed by the invention, a serum-free full-suspension cell culture is adopted, so that the method is not influenced by insufficient hatching eggs supply or uncontrollable factors; the method disclosed by the invention has the advantages of easy operation, large virus preparation amount and high virus content, and overcomes the defect that the supply of vaccines on the market is influenced by vaccine production stagnation caused by influence on virus reproduction due to insufficient supply of hatching eggs in virus chick embryo culture.
本发明涉及细胞和生物制品领域，具体涉及一种新城疫VII型弱毒株无血清全悬浮细胞培养的方法。在本发明所述方法中，所用新城疫病毒的毒株为新城疫基因VII型强毒经F基因位点突变而致弱的毒株。本发明所述方法，采用无血</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN112961837A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T20%3A00%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=WANG%20XIN&rft.date=2021-06-15&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN112961837A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |