Serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain

The invention relates to the field of cells and biological products, in particular to a serum-free full-suspension cell culture method for Newcastle disease VII type attenuated strain. In the method provided by the invention, the Newcastle disease virus strain is a Newcastle disease gene VII type virulent strain attenuated by F gene locus mutation. According to the method disclosed by the invention, a serum-free full-suspension cell culture is adopted, so that the method is not influenced by insufficient hatching eggs supply or uncontrollable factors; the method disclosed by the invention has the advantages of easy operation, large virus preparation amount and high virus content, and overcomes the defect that the supply of vaccines on the market is influenced by vaccine production stagnation caused by influence on virus reproduction due to insufficient supply of hatching eggs in virus chick embryo culture. 本发明涉及细胞和生物制品领域，具体涉及一种新城疫VII型弱毒株无血清全悬浮细胞培养的方法。在本发明所述方法中，所用新城疫病毒的毒株为新城疫基因VII型强毒经F基因位点突变而致弱的毒株。本发明所述方法，采用无血