Bispecific antibody targeting CD96 as well as preparation method and application of bispecific antibody

The invention belongs to the field of biological pharmacy, and particularly relates to a bispecific antibody targeting CD96 as well as a preparation method and application of the bispecific antibody. The bispecific antibody contains an anti-human CD96ScFv mutant, an anti-human PD1ScFv mutant and an IgG4Fc mutant, and the configuration of the bispecific antibody comprises CD96ScFv holes-PD1ScFv knobes and CD96ScFv-(G4S)4-PD1ScFv-Fc. The CD96 and PD-1 are combined for the first time, the CD96-PD1 bispecific antibody is constructed for the first time, the bispecific antibody has high affinity with the PD1 and the CD96, the CD96 is highly expressed in activated T cells and also highly expressed in activated NK cells, the expression quantity is increased along with the activation time, and the drug resistance problem caused by the single antibody of PD1 in the treatment process can be greatly reduced through the addition of the CD96. 本发明属于生物制药领域，具体涉及一种靶向CD96的双特异性抗体及其制备方法和用途。该双特异性抗体包含抗人CD96ScFv、抗人PD1ScFv和IgG4Fc突变体，