POLYMERIC TANDEM DYES HAVING PENDANT NARROW EMISSION ACCEPTOR

Polymeric tandem dyes are provided. The polymeric tandem dyes include a narrow emission fluorophore configured as a pendant acceptor in energy-receiving proximity to a donor water solvated light harvesting multichromophore. The narrow emission acceptor fluorophores of the present disclosure are capable of providing polymeric tandem dyes having a variety of narrow emission spectrums suitable for multiplexing. Also provided are compositions for multi-color fluorescent signal generation that include two or more polymeric tandem dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labeling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided. 本发明提供了聚合物串联染料。所述聚合物串联染料包括窄发射荧光团，所述窄发射荧光团被配置为在能量接收时接近供体水溶剂化捕光多发色团的侧基受体。本发明的窄发射受体荧光团能够提供聚合物串联染料，所述染料具有适用于多路复用的窄发射光谱。本发明还提供了用于多色荧光信号生成的组合物，所述组合物包括两种或更多种聚合物串联染料。此外，本发明还提供了评估样品中靶标分析物存在情况的方法以及标记靶分子的方法，其中在所述方法中使用标的聚合染料。本发明还提供用于实施标的方法的系统和试剂盒。