Method for acquiring relative abundance and activity of whole-course ammoxidation microorganisms from complex environment system based on macro omics technology

The invention belongs to the field of water and soil pollution prevention and control, and particularly relates to a method for acquiring relative abundance and activity of whole-course ammoxidation microorganisms from a complex environment system based on a macro omics technology. Through metagenom...

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Hauptverfasser: LIU SITONG, LIU SHUFENG, CHEN QIAN, WANG JIAWEN, NI JINREN, CAI HETONG, WANG HAIYING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of water and soil pollution prevention and control, and particularly relates to a method for acquiring relative abundance and activity of whole-course ammoxidation microorganisms from a complex environment system based on a macro omics technology. Through metagenome/metatranscriptome sequencing and bioinformatics processing, whole-course ammoxidation microbial genomes and transcripts are extracted from microbial communities with complex environments, the relative abundance, activity and community structure characteristics of whole-course ammoxidation microorganisms in a system are determined, a complete picture of a group metabolic potential model is constructed, and the potential contribution of the microorganisms to the nitrogen conversion process is determined. The method has the characteristics of high flux, high sensitivity and high accuracy, does not need to enrich and culture microorganisms, has an in-situ characteristic, is suitable for large-scale sampling investiga