Recombinant vector of giant panda rotavirus CH-1 strain VP7 protein, genetically engineered bacterium and application of recombinant vector and genetically engineered bacterium

The invention relates to the field of genetic engineering, and particularly discloses a recombinant vector of giant panda rotavirus CH-1 strain VP7 protein, a genetically engineered bacterium and application of the recombinant vector and the genetically engineered bacterium. The recombinant vector provided by the invention contains the coding gene of the panda rotavirus CH-1 strain VP7 protein. The giant panda rotavirus CH-1 strain VP7 protein expressed by the engineering bacteria containing the recombinant vector has good biological activity and immunogenicity, the protein expression quantity is large, the production cost is greatly reduced, and a foundation is laid for development of a detection kit of the giant panda rotavirus GPRV CH-1 strain. 本发明涉及基因工程领域，具体公开了一种大熊猫轮状病毒CH-1毒株VP7蛋白的重组载体、基因工程菌及其应用。本发明的重组载体，含有大熊猫轮状病毒CH-1毒株VP7蛋白的编码基因。含有本发明重组载体的工程菌表达的大熊猫轮状病毒CH-1毒株VP7蛋白具有良好的生物活性和免疫原性，蛋白表达量大，极大降低了生产成本，为大熊猫轮状病毒(giant panda rotavirus，GPRV)CH-1毒株的检测试剂盒的研制奠定了基础。