Antigen-specific Treg as well as preparation method and application thereof

The invention is applicable to the technical field of biology, and provides a preparation method and application of antigen-specific Treg, and the preparation method comprises the following steps: preparing a receptor tolDC; preparing a plasmid which contains all CDS region sequences of donor MHC-I molecule mRNA and is provided with a T7 promoter, and then preparing ivt-mRNA of the donor MHC-I molecule by applying an in-vitro transcription technology; the ivt-mRNA is transfected into the receptor tolDC, and the receptor tolDC for expressing and presenting the donor MHC-I molecular antigen is obtained; and co-culturing a receptor tolDC for expressing a donor MHC-I molecular antigen and a receptor nTreg cell under the condition of containing IL-2, so as to obtain the donor MHC-I molecular antigen specificity Treg. According to the preparation method, the receptor Treg cell with donor MHC-I antigen specificity and proper TCR-MHC antigen complex affinity can be prepared. 本发明适用于生物技术领域，提供了一种抗原特异性Treg的制备方法和应用，该制备方法包