Method for evaluating maternal and fetal immune tolerance by detecting level of human villus trophoblast exosome HLA-E

The invention discloses a method for evaluating maternal and fetal immune tolerance by detecting the level of human villus trophoblast exosome HLA-E. The method comprises the following steps: firstly, extracting human primary villus trophoblast exosome, and then analyzing the level of the exosome HLA-E through flow cytometry, thereby evaluating the level of maternal and fetal immune tolerance. The method needs less samples, is convenient to obtain, and is safe to patients; a flow cytometer is adopted for detection, so that the method is convenient and rapid; and the level of the exosome HLA-E can accurately reflect the degree of maternal and fetal immune tolerance. 本发明公开了一种检测人绒毛滋养细胞外泌体HLA-E水平评估母胎免疫耐受的方法，本发明首先提取出人原代绒毛滋养细胞外泌体，然后通过流式检测分析外泌体HLA-E的水平，从而评估母胎免疫耐受的水平。本发明方法需要的样本量少，获取方便，对病人安全；本发明采用流式细胞仪进行检测，方便快捷；外泌体HLA-E的水平可准确反映母胎免疫耐受的程度。