Construction method of porcine TFF1 gene knockout cell line based on CRISPR-Cas9 gene editing technology

The invention relates to a construction method of a porcine TFF1 gene knockout cell line based on a CRISPR-Cas9 gene editing technology. The TFF1 gene sequence in IPEC-J2 cells is knocked out by adopting the CRISPR/Cas9 technology, and the TFF1 gene knockout IPEC-J2 cell line is established and can...

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Bibliographische Detailangaben
Hauptverfasser:	BAO WENBIN, WANG HAIFEI, ZONG QIUFANG, QU HUAN, HUANG XIAOGUO, CAI DEMIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a construction method of a porcine TFF1 gene knockout cell line based on a CRISPR-Cas9 gene editing technology. The TFF1 gene sequence in IPEC-J2 cells is knocked out by adopting the CRISPR/Cas9 technology, and the TFF1 gene knockout IPEC-J2 cell line is established and can be used for functional verification of a TFF1 gene in porcine breeding for disease resistance, and an action mechanism of the TFF1 gene in disease resistance is disclosed. 一种基于CRISPR-Cas9基因编辑技术的猪TFF1基因敲除细胞系的构建方法，本发明采用CRISPR/Cas9技术敲除IPEC-J2细胞中的TFF1基因序列，建立TFF1基因敲除的IPEC-J2细胞系，可用于TFF1基因在猪抗病育种中的功能验证，揭示TFF1基因在疾病抗性中的作用机制。