Rice cryptochrome site-directed mutagenesis protein and construction method thereof

The invention discloses a rice cryptochrome site-directed mutagenesis protein and a construction method thereof. The construction method comprises the following steps: obtaining a gene sequence from a gene pool, carrying out artificial codon optimization and protein carboxyl terminal truncation to obtain a target gene sequence, and connecting the target gene sequence with a pET22b plasmid to construct a recombinant expression plasmid. The sequence of the truncated gene is as shown in SEQ ID NO:1. The construction method further comprises the following steps: designing mutation primers, carrying out site-directed mutagenesis by using the recombinant plasmid, and transforming the mutant recombinant plasmid into escherichia coli competent cells to construct genetically engineered bacteria. Compared with the prior art, the expression quantity of the expressed mutant enzyme S392L is higher, the polymerization form is tighter, and convenience is provided for in-vitro research. The S393L mutant enzyme provided by th