Lentivirus purification process

The invention discloses a lentivirus purification process. The lentivirus purification process comprises the following steps: S1, clarifying and filtering a lentivirus-containing cell culture to obtain a lentivirus-containing cell culture supernatant; S2, concentrating the supernatant obtained in the step S1 by 8-15 times by adopting a hollow fiber ultrafiltration system; S3, washing and filtering the concentrated solution obtained in the step S2 by at least 10 volumes by using a first buffer solution; S4, carrying out nuclease Benzonase enzyme digestion treatment on the concentrated solution washed and filtered in the step S3, and controlling the treatment temperature to be 25-37 DEG C and the treatment time to be 30-60 minutes; S5, washing and filtering the concentrated solution in the step S4 by at least 10 volumes through a hollow fiber ultrafiltration system so as to remove nuclease Benzonase and nucleic acid fragments, and then adding a second buffer solution into the concentrated solution, wherein the