Antler characteristic peptide fragment and detection method thereof

The invention discloses an antler characteristic peptide fragment and a detection method thereof. The antler characteristic peptide fragment Pep-G is screened and determined through a large amount of experimental screening, a label-free polypeptide quantification method and a principal component analysis and content comparison method, the amino acid sequence of the antler characteristic peptide fragment Pep-G is Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe-Lys, and the antler characteristic peptide fragment Pep-G can be used for distinguishing deer skin and antler. The method has the advantages of being strong in specificity, high in sensitivity and simple to operate, and can be used for distinguishing deer skin glue from antler glue and controlling quality. 本发明公开了一种鹿角特征肽段及其检测方法，本发明通过大量实验筛选，以非标记多肽定量方法、主成分分析及含量比较法，筛选确定一个鹿角特征肽段Pep-G，其氨基酸序列为：Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe-Lys，可用于区分鹿皮与鹿角。该方法专属性强、灵敏度高、操作简单，可用于鹿皮胶与鹿角胶的区分及质量控制。