METHODS FOR GENE MODIFICATION OF HEMATOPOIETIC CELLS

The present invention relates generally to methods for genetic modification of hematopoietic cells. In particular, the invention relates to use of Prostaglandin E2 (PGE2), poloxamer, and protamine sulfate to enhance transduction by a recombinant retroviral vector. The compositions and methods of the...

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Bibliographische Detailangaben
Hauptverfasser:	BEARD BRIAN, MESA NUNEZ CRISTINA, LAW KENNETH, SHAH GAURAV D, RIO GALDO PAULA, SEGOVIA SANZ JOSE CARLOS, BUEREN RONCERO JUAN ANTONIO, PATEL KINNARI, ALMARZA NOVOA ELENA, QUINTANA BUSTAMANTE OSCAR, NAVARRO ORDONEZ SUSANA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates generally to methods for genetic modification of hematopoietic cells. In particular, the invention relates to use of Prostaglandin E2 (PGE2), poloxamer, and protamine sulfate to enhance transduction by a recombinant retroviral vector. The compositions and methods of the present disclosure are particularly suitable for gene therapy applications, including the treatment of monogenic genetic diseases and disorders. 本发明总体上涉及用于造血细胞的基因修饰的方法。具体地，本发明涉及使用前列腺素E2(PGE2)、泊洛沙姆和硫酸鱼精蛋白来增强通过重组逆转录病毒载体进行的转导。本公开的组合物和方法特别适用于基因疗法应用，包含单基因遗传疾病和病症的治疗。