Antifreeze protein as well as preparation method and application thereof

The invention discloses antifreeze protein as well as a preparation method and application thereof, and belongs to the technical field of microbial antifreeze protein. According to the invention, a giant adhesion protein is found by analyzing the genome sequence of a bacterium BSI20414 separated fro...

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Hauptverfasser:	GAO SHANHUI, XU YI, LIAO LI, CHEN BO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses antifreeze protein as well as a preparation method and application thereof, and belongs to the technical field of microbial antifreeze protein. According to the invention, a giant adhesion protein is found by analyzing the genome sequence of a bacterium BSI20414 separated from Arctic sea ice, an antifreeze structural domain is predicted from the protein sequence, the structural domain sequence is cloned and expressed by using a genetic engineering means to obtain a soluble recombinant protein, and the activity research finds that the recombinant protein has high anti-freezing activity, has the function of inhibiting ice crystal growth and recrystallization, has application potential in the aspects of structure protection, cell protection and the like, and provides candidates for development of low-temperature protective agents. 本发明公开了一种抗冻蛋白及其制备方法和应用，属于微生物抗冻蛋白技术领域。本发明通过对从北极海冰中分离获得的细菌BSI20414基因组序列分析发现一个巨型粘附蛋白，从该蛋白序列中预测出一个抗冻结构域，利用基因工程手段对该结构域序列进行克隆表达，获得可溶性重组蛋白，经活性研究发现，该重组蛋白具有较高抗冻活性，具有抑制冰晶生