Method for extracting and purifying total flavonoids in albizia flowers
The invention discloses a method for extracting and purifying total flavonoids in albizia flowers. The method comprises the following steps: 1) freeze-drying the albizia flowers; 2) crushing the dried albizia flowers, and sieving with a 20-mesh sieve; 3) weighing albizia flower powder, adding ethano...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for extracting and purifying total flavonoids in albizia flowers. The method comprises the following steps: 1) freeze-drying the albizia flowers; 2) crushing the dried albizia flowers, and sieving with a 20-mesh sieve; 3) weighing albizia flower powder, adding ethanol, and performing ultrasonic-assisted extraction; 4) filtering the extracted material, and extracting and filtering the albizia flower powder cake; 5) pumping the albizia flower extract filtrate into a single-effect external circulation vacuum concentrator for vacuum concentration; (6) loading the obtained albizia flower extract concentrated solution on a macroporous resin column, eluting with distilled water and ethanol solutions with different concentrations respectively, and collecting an ethanol eluent with a certain concentration; and 7) carrying out vacuum concentration on the collected ethanol eluent; and the method fills the blank in the prior art, shortens the extraction, concentration and filtration time, |
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