Casuarina equisetifolia germplasm resource in-vitro preservation method

The invention discloses a casuarina equisetifolia germplasm resource in-vitro preservation method. The casuarina equisetifolia germplasm resource in-vitro preservation method comprises the following steps that tender and lignified branches of a casuarina equisetifolia plant are taken as explants, and the branches of casuarina equisetifolia are thoroughly cleaned; then sterilizing is carried out, and 1-3cm stem segments are cut from the sterilized branches of casuarina equisetifolia; the stem segments are inoculated into a starting culture medium for starting culture, and after 5-30 days, he stem segments are transferred into a propagation culture medium for propagation culture for 10-30 days; and then the stem segments are transferred into a preservation culture medium for preservation under the conditions that the temperature is 1-15 DEG C and the illumination is 200-1000Lux. According to the casuarina equisetifolia germplasm resource in-vitro preservation method, a sterile system for casuarina equisetifolia