Detection method for determining cGMP concentration in biological fluid sample by liquid chromatography-mass spectrometry

The invention belongs to the technical field of drug analysis, and relates to an analysis and determination method of in-vivo drugs, in particular to a detection method for determining the cGMP concentration in a biological fluid sample through liquid chromatography-mass spectrometry. The invention discloses a high-sensitivity method for determining the cAMP concentration in a biological fluid sample by liquid chromatography-mass spectrometry. The method comprises the following steps of: (1) pretreating a to-be-determined sample; (2) preparing a standard curve through liquid chromatography-mass spectrometry detection; and (3) detecting the concentration of cGMP in the to-be-determined sample by liquid chromatography-mass-spectrometry. The method is rapid, accurate and high in sensitivity, the sample pretreatment method is simple, repeatability is good, precision and accuracy are high, sensitivity is high, and the detection limit is low. 本发明属于药物分析技术领域，涉及体内药物的分析测定方法，具体涉及一种液质联用测定生物体液样品中cGMP浓度的检测方法。本发明公开了一种液质联用测定