Method for efficiently separating and culturing porcine mammary epithelial cells

The invention relates to a method for efficiently separating and culturing porcine mammary epithelial cells. The method comprises the steps of mammary tissue collection, pretreatment, mammary tissue block digestion, inoculation, cell purification, cell digestion passage and cell cryopreservation. According to the separation method, the porcine mammary epithelial cells are separated by using an enzyme digestion tissue block method, a porcine mammary epithelial cell line with stable growth is established, the time for separating the cells is short, the obtained cells are high in purity and do not need to be repeatedly purified, and the high-purity cells can be obtained only by purifying for 1-2 times; damage to cells is small, and the obtained cells are high in activity; and the cell growth is stable, the cell proliferation rate is not obviously changed when the cell is cultured to the tenth generation, the long-term experimental research is facilitated, and a stable experimental material is provided for the re