Method for detecting content of H9N2 subtype avian influenza virus by using digital PCR

The invention belongs to the technical field of microbial engineering, and particularly relates to a method for detecting the content of H9N2 subtype avian influenza virus by using digital PCR, which comprises the following steps: extracting DNA of HA gene and NA gene of the H9N2 subtype avian influenza virus in a sample to be detected to prepare a DNA template solution, preparing microdroplets together with primers and probes of the HA gene and NA gene designed in advance, then performing PCR amplification, and finally, detecting by using a microdroplet analyzer to obtain the copy numbers of the HA gene and the NA gene of the H9N2 subtype avian influenza virus. The method for detecting the copy number of the HA gene and the NA gene of the H9N2 subtype avian influenza virus is high in sensitivity, the content of the H9N2 subtype avian influenza virus can be detected through a digital PCR technology to optimize technical parameters of the serum-free full-suspension MDCK cell proliferation H9N2 subtype avian in