Efficient protein separation and purification method and device based on uniform mixing-liquid removal process

The invention relates to an efficient protein separation and purification method and device based on a uniform mixing-liquid removal process. The device is composed of an inner cylinder with holes, an outer cylinder and a variable-speed motor. wherein the motor drives the inner cylinder to rotate to generate centrifugal force, so that the Ni2+-loaded resin and the protein purification solution are subjected to solid-liquid separation, and the purpose of low-cost and high-efficiency preparation of the protein is achieved. The method can be suitable for separation and purification of specific binding of Ni2+-loaded resin and His tag protein, and can also be suitable for separation and purification of ion exchange particles and charged protein through electrostatic adsorption, wherein the cost of separating and purifying the protein is far lower than that of a chromatography method, and the defect that high-titer recombinant protein cannot be treated by a chromatography method can be overcome. A conventional chr