Method for extracting neomycin sulfate from neomycin fermentation liquor

The invention relates to the technical field of biology, in particular to a method for extracting neomycin sulfate from neomycin fermentation liquor. The invention relates to the method for extracting neomycin sulfate from neomycin fermentation liquor, which comprises the following steps of: (1) filtering the neomycin fermentation liquor, adsorbing by resin for the first time, washing, decolorizing and desorbing to obtain eluent; then concentrating the eluent to obtain a concentrated solution; (2) diluting the concentrated solution, adjusting the pH value to 6-7, and allowing the concentrated solution to flow into a cationic resin column for secondary adsorption; pre-washing and washing with first ammonia water and second ammonia water respectively; finally, analyzing by using third ammonia water, and collecting an analysis solution; and (3) concentrating the desorption solution, salifying, decolorizing with activated carbon, and drying to obtain the product. The method solves the problems of high content and