Method for amplifying target region of nucleic acid, library building and sequencing methods and kit

The invention relates to the field of gene sequencing, in particular to a method for amplifying a target area of nucleic acid and library building and sequencing methods. The provided method for amplifying the target region of the nucleic acid comprises the following steps of (1) inserting a UMI sequence into the nucleic acid by using transposase so as to obtain a fusion product which contains the UMI sequence and a target region sequence; and (2) amplifying the fusion product by using a primer so as to obtain an amplification product containing the target region sequence and the UMI sequence. The invention also provides the library building method for the target region of the nucleic acid and the sequencing method. According to the method provided by the invention, the time of library building and sequencing can be shortened; and the production cost can be reduced, and the data utilization rate is effectively improved. 本发明涉及基因测序领域，具体涉及一种对核酸的目标区域进行扩增的方法、建库和测序方法。所提供的对核酸的目标区域进行扩增的方法包括：(1)利用转座酶将UMI序列插入到所述核酸上，以便获