Method for solving limitation of depth of field of microscope

The invention discloses a method for solving the limitation of the depth of field of a microscope. The method comprises the following steps: S1, shooting a plurality of photos in different focal length ranges by using a camera; s2, pre-processing: converting the shot color picture into a grayscale image; s3, carrying out wavelet transformation; s4, fusion processing: for the low-frequency sub-images, obtaining a low-frequency fusion coefficient according to point sharpness and fusion measurement in a pixel neighborhood, and for the high-frequency sub-images, fusing according to the sum of Laplacian operators in the pixel neighborhood; s5, carrying out wavelet inverse transformation; and S6, converting the grayscale image in the S5 into a multi-channel picture, so that the problem that the depth of field is easily limited can be solved under the condition that the optical magnification factor of the optical microscope is relatively large, and the application scene of the optical microscope is greatly expanded.