Specific sequence for identifying NC297 baked variety, kit and use method

The invention relates to a specific sequence for identifying an NC297 baked variety, a kit and a use method, and belongs to the field of biomolecular identification. The kit comprises the specific sequence, a primer and a probe which are designed and synthesized according to the specific sequence, a...

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Hauptverfasser: ZHAO WENJUN, LI XIANGWEI, WANG AIGUO, JI DEHUI, HU BAOWEN, XUE CHAOQUN, DAI HUAXIN, SUN MENGMENG, YAO JIANGMEI, TIAN YANGYANG, YANG JIZHOU, LU JUNPING, XUAN BEIBEI, MOU WENJUN, WAN XIANG, HU LIWEI, WANG ZHENGXU, GUO JIANHUA, ZHU YUNCONG, WANG JUNWEI, LIU KUI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a specific sequence for identifying an NC297 baked variety, a kit and a use method, and belongs to the field of biomolecular identification. The kit comprises the specific sequence, a primer and a probe which are designed and synthesized according to the specific sequence, and a multi-channel ultra-low microfluidic nucleic acid detection chip for sensitively and intelligently controlling a flow path, a preloaded dry powder detection reagent and a positive quality control product. When the kit is used, different samples are added into the sample adding holes of the microfluidic nucleic acid detection chips, the sample adding holes are well covered, and microfluidic nucleic acid detection chips are detected in a microfluidic nucleic acid detection ship detector; and PCR amplification and result determination are performed. Compared with a conventional fluorescent PCR method and the like, the primer and the probe have the following advantages of high specificity against the design of the