Tissue culture method of oncidium hybridum

The invention relates to the technical field of plant tissue culture, and particularly discloses a tissue culture method of oncidium hybridum. The method comprises the following steps of S1, cutting a seedling with unexpanded leaves from a mother plant of the oncidium hybridum, cutting a terminal bud, soaking the terminal bud in alcohol, taking out the terminal bud, cutting a stem tip, and putting the stem tip in an induction culture medium for induction culture to obtain a protocorm; controlling the culture temperature and humidity, and using LED light for irradiating the stem tip in the induction culture period; S2, transferring the protocorm into a proliferation culture medium to be cultured, and obtaining buds; controlling the culture temperature and humidity, and using LED light for irradiating the protocorm during multiplication culture; S3, transferring the buds into a strong seedling culture medium, and culturing until the oncidium buds reach the length of 6-8cm, so as to obtain high-bud seedlings; an