Ophiocordyceps sinensis strain separation method and application thereof

The invention provides an ophiocordyceps sinensis strain separation method. The method comprises the following steps of 1) culturing fresh cordyceps sinensis under a sterile condition and collecting ejected cordyceps sinensis ascospores; 2) washing the cordyceps sinensis ascospores collected in the step 1) with sterile water under a sterile condition, and diluting to obtain an ascospore diluent; 3) coating and spreading a proper amount of the ascospore diluent obtained in the step 2) on a water agar plate, and culturing and germinating to form a mycelium group; and 4) selecting the mycelium group formed by germination of a single ascospore, transferring the mycelium group to a liquid culture medium for continuous culture, and inoculating the mycelium group to a new liquid culture medium or solid culture medium for amplification culture after the mycelium group generates a certain number of bud spores in the liquid culture medium to obtain ophiocordyceps sinensis. The invention also provides an artificial cult