Electrochemical detection method for protein based on molecular imprinting technology

The invention provides an electrochemical detection method for a protein based on a molecular imprinting technology. The method comprises the following steps: 1, preparing a required solution; 2, preparing UT-g-C3N4; 3, modifying a GCE electrode by using the UT-g-C3N4; 4, preparing MIPs/UT-g-C3N4/GC...

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Hauptverfasser: LIANG DONG, WANG RONGZHEN, XIANG-LIN HUIZI, GAO LI, CHENG CHEN, WANG HAIBIN, SHI NAN, GUO JIANFENG, WANG FANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an electrochemical detection method for a protein based on a molecular imprinting technology. The method comprises the following steps: 1, preparing a required solution; 2, preparing UT-g-C3N4; 3, modifying a GCE electrode by using the UT-g-C3N4; 4, preparing MIPs/UT-g-C3N4/GCE); and 5, putting all the electrodes into electrolyte solutions with the same concentration and under the same scanning condition to obtain a cyclic voltammetry curve, and performing comparative analysis. A two-dimensional nano material g-C3N4 is loaded as a matrix on a glassy carbon electrode, bovine serum albumin (BSA)is taken as a template molecule, acryloyloxyethyl trimethyl ammonium chloride (DAC) is taken as a functional monomer, and N, N' -methylene bisacrylamide (MBA) is taken as a cross-linking agent, a molecular imprinting electrochemical sensor capable of specifically recognizing BSA molecules is prepared in an aqueous solution by using the novel surface molecular imprinting technology, and a target pro