Coronavirus pseudovirus packaging system and packaging method, and application of coronavirus pseudovirus to evaluating disinfection efficacy

The invention relates to a coronavirus pseudovirus packaging system. The coronavirus pseudovirus packaging system comprises a vesicular stomatitis virus VSV vector and an assembly cell, wherein the vesicular stomatitis virus VSV vector is formed by replacing GP genes with Fluc and EGFP double reporter genes, and the assembly cell is used for expressing coronavirus spike protein S. The double reporter genes are selected from luciferase and fluorescent protein, and the luciferase reporter gene is preferably the Fluc gene. According to the packaging system, a one-step packaging method is adopted, so that pseudoviruses which are infected in a single cycle, low in background value and high in titer and have the characteristic of rapid detection compared with a lentivirus-mediated pseudovirus system can be rapidly packaged, and the packaging system can be used for researching coronaviruses such as COVID-19 (SARS-CoV-2), SARS (SARS-CoV) and MERS; and the pseudoviruses can be used for evaluating the efficacy of a dis