Method and kit for detecting catecholamine and metabolite in blood plasma
The invention provides a method and a kit for detecting catecholamine and metabolite in blood plasma. The method comprises the following steps: (a) respectively pretreating a standard substance solution and a plasma sample to be detected by using a precipitant; (b) deriving the plasma sample pretrea...
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| creator | MA LIANG KONG HONGWEI ZHENG XIANMIN OUYANG XIEHUA YU SHUXIN CHEN JIA |
| description | The invention provides a method and a kit for detecting catecholamine and metabolite in blood plasma. The method comprises the following steps: (a) respectively pretreating a standard substance solution and a plasma sample to be detected by using a precipitant; (b) deriving the plasma sample pretreated in the step (a) by using a derivation reagent I without isotope labeling to obtain a plasma derived product; (c) deriving the standard substance solution pretreated in the step (a) by using an isotope labeled derivative reagent II to obtain a standard substance derivative product; and (d) mixing the plasma derivative product derivatized in the step (b) with the standard substance derivative product derivatized in the step (c), and carrying out separation and detection by virtue of ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry. The detection method is low in cost, small in required sample size, short in detection time and high in analysis flux.
本发明提供了一种用于检测血浆中儿茶酚胺及代谢物的方法 |
| format | Patent |
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本发明提供了一种用于检测血浆中儿茶酚胺及代谢物的方法</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPD0TS3JyE9RSMxLUcjOLFFIyy9SSEktSU0uycxLV0hOBLIy8nMSczPzUsFqclNLEpPyczJLUhUy8xSScvKBegtyEotzE3kYWNMSc4pTeaE0N4Oim2uIs4duakF-fGpxQWJyal5qSbyzn6GhkbmxgZmlpaMxMWoAsns0YQ</recordid><startdate>20210430</startdate><enddate>20210430</enddate><creator>MA LIANG</creator><creator>KONG HONGWEI</creator><creator>ZHENG XIANMIN</creator><creator>OUYANG XIEHUA</creator><creator>YU SHUXIN</creator><creator>CHEN JIA</creator><scope>EVB</scope></search><sort><creationdate>20210430</creationdate><title>Method and kit for detecting catecholamine and metabolite in blood plasma</title><author>MA LIANG ; KONG HONGWEI ; ZHENG XIANMIN ; OUYANG XIEHUA ; YU SHUXIN ; CHEN JIA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112730699A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>MA LIANG</creatorcontrib><creatorcontrib>KONG HONGWEI</creatorcontrib><creatorcontrib>ZHENG XIANMIN</creatorcontrib><creatorcontrib>OUYANG XIEHUA</creatorcontrib><creatorcontrib>YU SHUXIN</creatorcontrib><creatorcontrib>CHEN JIA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MA LIANG</au><au>KONG HONGWEI</au><au>ZHENG XIANMIN</au><au>OUYANG XIEHUA</au><au>YU SHUXIN</au><au>CHEN JIA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method and kit for detecting catecholamine and metabolite in blood plasma</title><date>2021-04-30</date><risdate>2021</risdate><abstract>The invention provides a method and a kit for detecting catecholamine and metabolite in blood plasma. The method comprises the following steps: (a) respectively pretreating a standard substance solution and a plasma sample to be detected by using a precipitant; (b) deriving the plasma sample pretreated in the step (a) by using a derivation reagent I without isotope labeling to obtain a plasma derived product; (c) deriving the standard substance solution pretreated in the step (a) by using an isotope labeled derivative reagent II to obtain a standard substance derivative product; and (d) mixing the plasma derivative product derivatized in the step (b) with the standard substance derivative product derivatized in the step (c), and carrying out separation and detection by virtue of ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry. The detection method is low in cost, small in required sample size, short in detection time and high in analysis flux.
本发明提供了一种用于检测血浆中儿茶酚胺及代谢物的方法</abstract><oa>free_for_read</oa></addata></record> |
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| title | Method and kit for detecting catecholamine and metabolite in blood plasma |
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