Micro-droplet type digital PCR primer, probe, kit and method for quantitatively detecting saffron

The invention belongs to the field of molecular biological detection, and discloses a droplet type digital PCR primer, a probe, a kit and a method for quantitatively detecting saffron. The nucleotide sequence of the microdroplet type digital PCR primer for quantitative detection of the saffron is XH...

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Hauptverfasser:	LIN WEI, SHUI KEJUAN, QUAN YONGBING, ZHANG WEIDONG, LIAO LI, XU MIAOFENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of molecular biological detection, and discloses a droplet type digital PCR primer, a probe, a kit and a method for quantitatively detecting saffron. The nucleotide sequence of the microdroplet type digital PCR primer for quantitative detection of the saffron is XHH-ITS-F: 5'-ATAAGCGCGCCCGAGAGATGAT-3' (SEQ ID No.1), and the nucleotide sequence of the microdroplet type digital PCR probe for quantitative detection of the saffron is XHH-ITS-R: 5'-CATCCTGGGGTCGCATTCGAT-3' (SEQ ID No.2). The nucleotide sequence of the microdroplet type digital PCR probe for quantitative detection of the saffron is XHH-ITS-P: 5'-CGCCATCCATGGATTAGCGCATGGA-3' (SEQ ID No.3). By adopting the primer and the probe, quantitative detection of the saffron can be carried out, and the microdroplet type digital PCR probe has good specificity and sensitivity. 本发明属于分子生物学检测领域，公开了一种定量检测西红花的微滴式数字PCR引物、探针、试剂盒及方法。所述定量检测西红花的微滴式数字PCR引物的核苷酸序列为：XHH-ITS-F：5'-ATAAGCGCGCCCGAGAGATGAT-3'(SEQ ID No.1)，XHH-ITS-R：5'-CATCCTGGGGT