Artificially modified AKT3 circular RNA and application thereof in new crown vaccine

The invention discloses an artificially modified AKT3 circular RNA (ribonucleic acid) and application thereof in a new crown vaccine, which are characterized in that the modified AKT3 circular RNA has a transcription starting function, a section of protein open reading frame is added in the middle, so that the circular RNA can stably express the protein of the protein open reading frame, and if a corresponding immunogen protein open reading frame is added, the protein of the protein open reading frame can be stably expressed. The monoclonal antibody can act on a carrier of a circRNA vaccine, so that an immune animal generates an antibody, and the monoclonal antibody can be used as a vaccine for prevention or treatment. 本发明公开了一种人工改造的AKT3环状RNA及其在新冠疫苗中的应用，其特征是：经过改造AKT3环状RNA具有启动转录功能，在中间加入一段蛋白开放阅读框，使该环状RNA可稳定的表达上述白开放阅读框的蛋白，如果加入相应的免疫原蛋白开放阅读框，可以作用circRNA疫苗的载体，使免疫动物产生抗体，可用于为预防或治疗的疫苗。