Trichothecene-resistant self-protection gene GNAT11 of Leucosphaeria dothidea A553 and application thereof

The invention discloses a Myrothecium roridum A553 anti-trichothecene self-protection gene GNAT1 and an application thereof. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. Based on a transcriptome sequencing result, a cDNA library is used as a template, and amplification is perform...

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Hauptverfasser: LI SAINI, ZHU MUZI, CEN YOUFEI, YE WEI, ZHANG WEIMIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a Myrothecium roridum A553 anti-trichothecene self-protection gene GNAT1 and an application thereof. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. Based on a transcriptome sequencing result, a cDNA library is used as a template, and amplification is performed to obtain the Myrothecium roridum A553 anti-trichothecene self-protection gene GNAT1. The GNAT1 obtained through amplification is inserted into a saccharomyces cerevisiae expression vector and then converted into saccharomyces cerevisiae, the tolerance of the saccharomyces cerevisiae to trichothecene toxins can be remarkably improved, and therefore a molecular biological strategy is provided for plants to resist plant pathogenic fungi in the later period; meanwhile, a molecular biological foundation is laid for increasing the yield of trichothecene toxins through heterologous expression in the later period and obtaining novel trichothecene compounds. 本发明公开了一种露湿漆斑菌Myrothecium roridum A553抗单端孢霉烯自我保护基因GNAT1及其应用。该基因