Recombinant salt-tolerant adenylate cyclase as well as coding gene and application thereof

Recombinant salt-tolerant adenylate cyclase as well as coding gene and application thereof

The invention discloses arecombinant salt-tolerant adenylate cyclase as well as a coding gene and an application thereof. The nucleotide sequence of the recombinant salt-tolerant adenylate cyclase is shown as SEQ ID NO.1, the length of the recombinant salt-tolerant adenylate cyclase is 1008 bp, the...

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Bibliographische Detailangaben
Hauptverfasser: NIU HUANQING, LIU DONG, YING HANJIE, CHEN YONG, JIANG DAHAI, YU BIN, ZHAO GULIN
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses arecombinant salt-tolerant adenylate cyclase as well as a coding gene and an application thereof. The nucleotide sequence of the recombinant salt-tolerant adenylate cyclase is shown as SEQ ID NO.1, the length of the recombinant salt-tolerant adenylate cyclase is 1008 bp, the amino acid sequence of the recombinant salt-tolerant adenylate cyclase is shown as SEQ ID NO.2, the recombinant salt-tolerant adenylate cyclase has 335 amino acids in total, and the theoretical molecular weight of the recombinant salt-tolerant adenylate cyclase is 36.09 kDa. The optimum pH of the salt-tolerant adenylate cyclase is 10.0, the optimum temperature is 45 DEG C, the activity of the recombinant salt-tolerant adenylate cyclase is enhanced along with the increase of the concentration of sodium chloride, the enzyme activity reaches the maximum (about 22 times) when the concentration of sodium chloride is 2.5 M, and the salt tolerance is good. Compared with the currently reported adenylate cyclase, the recomb