Porcine oocyte in-vitro maturation culture solution with polyspermia inhibition effect as well as preparation method and application thereof

The invention provides a porcine oocyte in-vitro maturation culture solution with an effect of inhibiting polyspermia fertilization as well as a preparation method and an application of the porcine oocyte in-vitro maturation culture solution, and belongs to the technical field of livestock raising. In order to effectively inhibit the occurrence of polyspermia fertilization, the later embryo development quality is ensured. The porcine oocyte in-vitro maturation culture solution with the polyspermia inhibition effect is prepared from 0.6 mmol/L cysteine, 0.91 mmol/L sodium pyruvate, 10ng/mL EGF, 10% porcine follicular fluid, 10IU/mL FSH, 10IU/mL LH, 1% penicillin and 10-11-10-5mol/L melatonin based on TCM199. According to the melatonin-containing porcine oocyte in-vitro maturation culture solution, the in-vitro maturation quality of porcine oocytes is improved by promoting cytoplasm maturation of the porcine oocytes, so that the polyspermia fertilization effect of the oocytes in the in-vitro fertilization proce