Preparation type isoelectric point electrophoresis separating method and equipment

Between the two long narrow parallel electrodes, the gel film is placed along the length direction, and the space between electrodes is divided into the middle separating chamber and two sides flushing chambers, and in the separating chamber the sample solution to be separated whose pH has been regulated to the isoelectric point (PI value) of the object product is introduced, and in the flushing chambers the ordinary buffer solution whose pH is the isoelectric point of the object product respectively is introduced, under the action of electric field, the impurity protein whose isoelectric point differs from that of the object product is migrated into the flushing chambers at two sides and then eluted out, thus the separation is implemented.