Kit as well as preparation method and application thereof

The invention discloses a kit as well as a preparation method and application thereof, and belongs to the technical field of drug detection. The kit is used for detecting the content of beta human chorionic gonadotropin. The kit comprises an R1 reagent, an R2 reagent and an R3 reagent; the R1 reagent comprises streptavidin-coated magnetic beads and a first buffer solution with a pH value of 7.2-7.6; the R2 reagent comprises an enzyme labeled beta human chorionic gonadotropin monoclonal antibody and a second buffer solution with the pH value of 6.3-6.7; and the R3 reagent comprises a biotin labeled beta human chorionic gonadotropin monoclonal antibody and a third buffer solution with the pH value of 7.2-7.6. When the kit provided by the invention is used for detecting the content of beta human chorionic gonadotropin, the sensitivity is high, and the stability and the repeatability are good. 本发明公开一种试剂盒及其制备方法和应用，涉及药物检测技术领域，所述试剂盒用于检测β人绒毛膜促性腺激素的含量，包括：R1试剂，包括包被有链霉亲和素的磁珠以及pH值为7.2～7.6的第一缓冲液；R2试剂，包括酶标记的β人绒毛膜促性腺激素单克隆抗体