Preparation method of three-dimensional graphene modified electrode for detecting phospholipid in crude oil and electrode

The invention relates to the field of electrochemical biosensors, and discloses a preparation method of a three-dimensional graphene modified electrode for detecting phospholipid in crude oil and the electrode. The preparation method of the modified electrode comprises the following steps of firstly, preparing three-dimensional graphene by adopting a one-step thermal reduction method, crosslinking horse radish peroxidase on the surface of the three-dimensional graphene modified screen-printed electrode by utilizing glutaraldehyde, then adding chitosan on the surface, and then crosslinking choline oxidase on the surface of the electrode by adopting glutaraldehyde, and constructing the three-dimensional graphene/double-enzyme modified biological enzyme electrode. The obtained modified electrode is used as a working electrode, a silver/silver chloride electrode is used as a reference electrode, a three-dimensional graphene/double-enzyme modified bio-enzyme electrode is used as a counter electrode, a three-electr