Preparation method and detection method of three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil

Preparation method and detection method of three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil

The invention relates to the field of photoelectrochemical biosensors, and discloses a preparation method and a detection method of a three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil. Firstly, three-dimensional SnO2 nanoflowers are synthesized th...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: YAO JING, SUI YULIN, WANG LIQI, SONG YANG, LUO SHUNIAN, WANG RUIYING, YU DIANYU
Format: Patent
Sprache:chi ; eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator YAO JING
SUI YULIN
WANG LIQI
SONG YANG
LUO SHUNIAN
WANG RUIYING
YU DIANYU
description The invention relates to the field of photoelectrochemical biosensors, and discloses a preparation method and a detection method of a three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil. Firstly, three-dimensional SnO2 nanoflowers are synthesized through a hydrothermal method, a polythionine modified SnO2 nanoflower photosensitive electrode is prepared through an electropolymerization method, choline oxidase is modified on the surface of the polythionine modified SnO2 nanoflower photosensitive electrode through a cross-linking method, and then a double-enzyme modified photosensitive electrode is constructed through glutaraldehyde cross-linked phospholipase D; the modified photosensitive electrode has good selectivity and high sensitivity. The obtained modified photosensitive electrode is used as a working electrode, a silver/silver chloride electrode is used as a reference electrode, a platinum electrode is used as a counter electrode, a three-electrode syste
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN112710716A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN112710716A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN112710716A3</originalsourceid><addsrcrecordid>eNqNjLEKwjAYhLs4iPoOvw9QsAo6S1GcxMG9pMnVBJL8IUkHfQvf2AgdHB2Og7vvbl69bxFBRJENe3LImhUJr0ghQ_6GPFDWEaiVcfCpNMKS4rG3qOFfTwcKmjPDll1kqeGMLEhvOBWeIw1F061_fOFUZE0wiownGUcFYmOX1WwQNmE1-aJan0_39lIjcIcUhIRH7tpr02wPzebQ7I-7f5gPzdhREw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Preparation method and detection method of three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil</title><source>esp@cenet</source><creator>YAO JING ; SUI YULIN ; WANG LIQI ; SONG YANG ; LUO SHUNIAN ; WANG RUIYING ; YU DIANYU</creator><creatorcontrib>YAO JING ; SUI YULIN ; WANG LIQI ; SONG YANG ; LUO SHUNIAN ; WANG RUIYING ; YU DIANYU</creatorcontrib><description>The invention relates to the field of photoelectrochemical biosensors, and discloses a preparation method and a detection method of a three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil. Firstly, three-dimensional SnO2 nanoflowers are synthesized through a hydrothermal method, a polythionine modified SnO2 nanoflower photosensitive electrode is prepared through an electropolymerization method, choline oxidase is modified on the surface of the polythionine modified SnO2 nanoflower photosensitive electrode through a cross-linking method, and then a double-enzyme modified photosensitive electrode is constructed through glutaraldehyde cross-linked phospholipase D; the modified photosensitive electrode has good selectivity and high sensitivity. The obtained modified photosensitive electrode is used as a working electrode, a silver/silver chloride electrode is used as a reference electrode, a platinum electrode is used as a counter electrode, a three-electrode syste</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20210427&amp;DB=EPODOC&amp;CC=CN&amp;NR=112710716A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20210427&amp;DB=EPODOC&amp;CC=CN&amp;NR=112710716A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YAO JING</creatorcontrib><creatorcontrib>SUI YULIN</creatorcontrib><creatorcontrib>WANG LIQI</creatorcontrib><creatorcontrib>SONG YANG</creatorcontrib><creatorcontrib>LUO SHUNIAN</creatorcontrib><creatorcontrib>WANG RUIYING</creatorcontrib><creatorcontrib>YU DIANYU</creatorcontrib><title>Preparation method and detection method of three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil</title><description>The invention relates to the field of photoelectrochemical biosensors, and discloses a preparation method and a detection method of a three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil. Firstly, three-dimensional SnO2 nanoflowers are synthesized through a hydrothermal method, a polythionine modified SnO2 nanoflower photosensitive electrode is prepared through an electropolymerization method, choline oxidase is modified on the surface of the polythionine modified SnO2 nanoflower photosensitive electrode through a cross-linking method, and then a double-enzyme modified photosensitive electrode is constructed through glutaraldehyde cross-linked phospholipase D; the modified photosensitive electrode has good selectivity and high sensitivity. The obtained modified photosensitive electrode is used as a working electrode, a silver/silver chloride electrode is used as a reference electrode, a platinum electrode is used as a counter electrode, a three-electrode syste</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjLEKwjAYhLs4iPoOvw9QsAo6S1GcxMG9pMnVBJL8IUkHfQvf2AgdHB2Og7vvbl69bxFBRJENe3LImhUJr0ghQ_6GPFDWEaiVcfCpNMKS4rG3qOFfTwcKmjPDll1kqeGMLEhvOBWeIw1F061_fOFUZE0wiownGUcFYmOX1WwQNmE1-aJan0_39lIjcIcUhIRH7tpr02wPzebQ7I-7f5gPzdhREw</recordid><startdate>20210427</startdate><enddate>20210427</enddate><creator>YAO JING</creator><creator>SUI YULIN</creator><creator>WANG LIQI</creator><creator>SONG YANG</creator><creator>LUO SHUNIAN</creator><creator>WANG RUIYING</creator><creator>YU DIANYU</creator><scope>EVB</scope></search><sort><creationdate>20210427</creationdate><title>Preparation method and detection method of three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil</title><author>YAO JING ; SUI YULIN ; WANG LIQI ; SONG YANG ; LUO SHUNIAN ; WANG RUIYING ; YU DIANYU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112710716A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>YAO JING</creatorcontrib><creatorcontrib>SUI YULIN</creatorcontrib><creatorcontrib>WANG LIQI</creatorcontrib><creatorcontrib>SONG YANG</creatorcontrib><creatorcontrib>LUO SHUNIAN</creatorcontrib><creatorcontrib>WANG RUIYING</creatorcontrib><creatorcontrib>YU DIANYU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YAO JING</au><au>SUI YULIN</au><au>WANG LIQI</au><au>SONG YANG</au><au>LUO SHUNIAN</au><au>WANG RUIYING</au><au>YU DIANYU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Preparation method and detection method of three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil</title><date>2021-04-27</date><risdate>2021</risdate><abstract>The invention relates to the field of photoelectrochemical biosensors, and discloses a preparation method and a detection method of a three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil. Firstly, three-dimensional SnO2 nanoflowers are synthesized through a hydrothermal method, a polythionine modified SnO2 nanoflower photosensitive electrode is prepared through an electropolymerization method, choline oxidase is modified on the surface of the polythionine modified SnO2 nanoflower photosensitive electrode through a cross-linking method, and then a double-enzyme modified photosensitive electrode is constructed through glutaraldehyde cross-linked phospholipase D; the modified photosensitive electrode has good selectivity and high sensitivity. The obtained modified photosensitive electrode is used as a working electrode, a silver/silver chloride electrode is used as a reference electrode, a platinum electrode is used as a counter electrode, a three-electrode syste</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN112710716A
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Preparation method and detection method of three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T21%3A56%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=YAO%20JING&rft.date=2021-04-27&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN112710716A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true