Preparation method and detection method of three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil

The invention relates to the field of photoelectrochemical biosensors, and discloses a preparation method and a detection method of a three-dimensional double-enzyme photoelectrochemical biosensor for detecting phospholipid in crude oil. Firstly, three-dimensional SnO2 nanoflowers are synthesized through a hydrothermal method, a polythionine modified SnO2 nanoflower photosensitive electrode is prepared through an electropolymerization method, choline oxidase is modified on the surface of the polythionine modified SnO2 nanoflower photosensitive electrode through a cross-linking method, and then a double-enzyme modified photosensitive electrode is constructed through glutaraldehyde cross-linked phospholipase D; the modified photosensitive electrode has good selectivity and high sensitivity. The obtained modified photosensitive electrode is used as a working electrode, a silver/silver chloride electrode is used as a reference electrode, a platinum electrode is used as a counter electrode, a three-electrode syste