Primer pair and kit for identifying wasabi, horseradish and mustard components in mustard condiment and application of primer pair and kit

The invention discloses a primer pair and kit for identifying wasabi, horseradish and mustard components in a mustard condiment and application of the primer pair and the kit, and belongs to the technical field of food detection. The method for identifying the wasabi, horseradish and mustard components in the mustard condiment by utilizing ITS2 can be used for identifying complex components in the green mustard condiment and identifying whether a green mustard product contains wasabi, horseradish or mustard. Besides, the method can also be applied to real-time quantitative PCR, so that the content of each component in a sample containing the components such as wasabi, horseradish and mustard can be detected at the same time, and a more efficient and sensitive detection method is provided for green mustard related food detection. 本发明公开了一种用于鉴定芥辣调味品中山葵、辣根和芥末组分的引物对、试剂盒及其应用，属于食品检测技术领域。利用ITS2鉴定芥辣调味品中山葵、辣根和芥末组分的方法可以鉴别青芥辣调味品中复杂成分，鉴定青芥辣产品中是否含有山葵、辣根或芥末。另外，还可以将该方法应用于实时定量PCR，从而同时检测出含有山葵、辣根、芥末等成分的样品中各成分的含量，为青芥辣相关食品检测提供一种更