Primer pair and probe for detecting chicken-derived components as well as kit and application of kit

The invention discloses a primer pair and probe for detecting chicken-derived components, and discloses a kit designed according to the primer pair and probe. The kit comprises an RAA reaction system and an LFD test strip, wherein the RAA reaction system comprises general dry powder for an RAA react...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	WANG ZHONGHE, LIN MIN, HUANG HUIYING, KONG MEILAN, ZHENG YUZHONG, YANG PEIKUI, LIN LIYUN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a primer pair and probe for detecting chicken-derived components, and discloses a kit designed according to the primer pair and probe. The kit comprises an RAA reaction system and an LFD test strip, wherein the RAA reaction system comprises general dry powder for an RAA reaction, a Tris-HCl buffer solution, an upstream primer JI-F, a downstream primer JI-R, a probe JI-PRO, a sample DNA extracting solution, MgAcO and ddH2O; a quality control line of the LFD test strip is provided with an avidin-colloidal gold specific antibody; and a detection line of the LFD test strip is provided with a biotin antibody and a fluorophore antibody. Based on a recombinase-mediated isothermal nucleic acid amplification technology and a lateral flow immunoassay technology, the invention provides a novel chicken-derived component adulteration on-site detection method; visual detection of naked eye results can be realized; and compared with other detection methods, the method is high in detection sensitivity