Preparation method of Pyrophen compound

The invention discloses a preparation method of a Pyrophen compound. The preparation method comprises the following steps of taking a Pholiota nameko strain, preparing a seed solution by using a liquid culture medium, carrying out enlarged fermentation through solid fermentation, extracting a fermentation product with ethyl acetate for three times, concentrating obtained extracting liquid to obtain extractum, carrying out RP-18 column chromatography, silica gel column chromatography and Sephedex LH-20 column chromatography on the extractum, and carrying out elution separation by using different solvents, thereby obtaining the Pyrophen compound. The compound is extracted from a large fungus Pholiota nameko fermentation broth, and the compound and a pharmaceutical composition thereof are applied to preparation of drugs for treating and preventing tumors and pathogenic bacteria infection. 本发明公开一种Pyrophen化合物的制备方法，取滑菇(Pholiota nameko)菌种，采用液体培养基制备种子液，通过固体发酵进行扩大发酵，发酵产物用乙酸乙酯萃取3次，所得萃取液浓缩得浸膏，浸膏经RP-18柱层析、硅胶柱层析和Sephedex