Method for increasing raw material utilization rate of aureomycin fermentation tank
The invention belongs to the technical field of aureomycin fermentation, and particularly discloses a method for increasing the raw material utilization rate of an aureomycin fermentation tank. Aureomycin fermentation comprises first-stage fermentation, second-stage fermentation and third-stage ferm...
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| creator | YUAN WANLI CAO HUAWEI LI XUECHENG LYU XIANGYUN ZHANG HUAN SUN QINGFENG QU YANFANG |
| description | The invention belongs to the technical field of aureomycin fermentation, and particularly discloses a method for increasing the raw material utilization rate of an aureomycin fermentation tank. Aureomycin fermentation comprises first-stage fermentation, second-stage fermentation and third-stage fermentation; compound protease is added in the third-stage fermentation stage; the compound protease is prepared by mixing papain, neutral protease, acid protease and trypsin according to a ratio of 4:2:2:1; furthermore, the addition amount of the compound protease is 0.1-0.5% of the weight of a nitrogen source in a culture medium in the three-stage fermentation stage; the activity of the compound protease is 200,000 U/g; by adding the compound protease, the delayed nitrogen source is reduced by 10%; and the fermentation titer is improved by 6%.
本发明属于金霉素发酵技术领域，具体公开一种提高金霉素发酵罐原料利用率的方法，金霉素发酵包括一级发酵、二级发酵和三级发酵，在三级发酵阶段添加复合蛋白酶，所述复合蛋白酶为木瓜蛋白酶、中性蛋白酶、酸性蛋白酶和胰蛋白酶按照4:2:2:1比例混合配制而成，且复合蛋白酶的添加量为三级发酵阶段培养基中氮源重量的0.1-0.5%，复合蛋白酶活性为20万U/g，通过 |
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本发明属于金霉素发酵技术领域，具体公开一种提高金霉素发酵罐原料利用率的方法，金霉素发酵包括一级发酵、二级发酵和三级发酵，在三级发酵阶段添加复合蛋白酶，所述复合蛋白酶为木瓜蛋白酶、中性蛋白酶、酸性蛋白酶和胰蛋白酶按照4:2:2:1比例混合配制而成，且复合蛋白酶的添加量为三级发酵阶段培养基中氮源重量的0.1-0.5%，复合蛋白酶活性为20万U/g，通过</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyk0OAUEQQOHZWAjuUA4gMX4mLGVCbNiwn1RaNRXdVZPumginJ-EAVm_xvX5xOpDd9AJeE7C4RJhZrpDwARGNEmOAzjjwC41VPmAE6gG7RBqfjgU8pUhiXzeU-7DoeQyZRr8OivFue673E2q1odyiIyFr6mNZzqrVdLFebub_PG-hAzkZ</recordid><startdate>20210420</startdate><enddate>20210420</enddate><creator>YUAN WANLI</creator><creator>CAO HUAWEI</creator><creator>LI XUECHENG</creator><creator>LYU XIANGYUN</creator><creator>ZHANG HUAN</creator><creator>SUN QINGFENG</creator><creator>QU YANFANG</creator><scope>EVB</scope></search><sort><creationdate>20210420</creationdate><title>Method for increasing raw material utilization rate of aureomycin fermentation tank</title><author>YUAN WANLI ; CAO HUAWEI ; LI XUECHENG ; LYU XIANGYUN ; ZHANG HUAN ; SUN QINGFENG ; QU YANFANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112680495A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YUAN WANLI</creatorcontrib><creatorcontrib>CAO HUAWEI</creatorcontrib><creatorcontrib>LI XUECHENG</creatorcontrib><creatorcontrib>LYU XIANGYUN</creatorcontrib><creatorcontrib>ZHANG HUAN</creatorcontrib><creatorcontrib>SUN QINGFENG</creatorcontrib><creatorcontrib>QU YANFANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YUAN WANLI</au><au>CAO HUAWEI</au><au>LI XUECHENG</au><au>LYU XIANGYUN</au><au>ZHANG HUAN</au><au>SUN QINGFENG</au><au>QU YANFANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for increasing raw material utilization rate of aureomycin fermentation tank</title><date>2021-04-20</date><risdate>2021</risdate><abstract>The invention belongs to the technical field of aureomycin fermentation, and particularly discloses a method for increasing the raw material utilization rate of an aureomycin fermentation tank. Aureomycin fermentation comprises first-stage fermentation, second-stage fermentation and third-stage fermentation; compound protease is added in the third-stage fermentation stage; the compound protease is prepared by mixing papain, neutral protease, acid protease and trypsin according to a ratio of 4:2:2:1; furthermore, the addition amount of the compound protease is 0.1-0.5% of the weight of a nitrogen source in a culture medium in the three-stage fermentation stage; the activity of the compound protease is 200,000 U/g; by adding the compound protease, the delayed nitrogen source is reduced by 10%; and the fermentation titer is improved by 6%.
本发明属于金霉素发酵技术领域，具体公开一种提高金霉素发酵罐原料利用率的方法，金霉素发酵包括一级发酵、二级发酵和三级发酵，在三级发酵阶段添加复合蛋白酶，所述复合蛋白酶为木瓜蛋白酶、中性蛋白酶、酸性蛋白酶和胰蛋白酶按照4:2:2:1比例混合配制而成，且复合蛋白酶的添加量为三级发酵阶段培养基中氮源重量的0.1-0.5%，复合蛋白酶活性为20万U/g，通过</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE |
| title | Method for increasing raw material utilization rate of aureomycin fermentation tank |
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