Method for increasing raw material utilization rate of aureomycin fermentation tank

The invention belongs to the technical field of aureomycin fermentation, and particularly discloses a method for increasing the raw material utilization rate of an aureomycin fermentation tank. Aureomycin fermentation comprises first-stage fermentation, second-stage fermentation and third-stage fermentation; compound protease is added in the third-stage fermentation stage; the compound protease is prepared by mixing papain, neutral protease, acid protease and trypsin according to a ratio of 4:2:2:1; furthermore, the addition amount of the compound protease is 0.1-0.5% of the weight of a nitrogen source in a culture medium in the three-stage fermentation stage; the activity of the compound protease is 200,000 U/g; by adding the compound protease, the delayed nitrogen source is reduced by 10%; and the fermentation titer is improved by 6%. 本发明属于金霉素发酵技术领域，具体公开一种提高金霉素发酵罐原料利用率的方法，金霉素发酵包括一级发酵、二级发酵和三级发酵，在三级发酵阶段添加复合蛋白酶，所述复合蛋白酶为木瓜蛋白酶、中性蛋白酶、酸性蛋白酶和胰蛋白酶按照4:2:2:1比例混合配制而成，且复合蛋白酶的添加量为三级发酵阶段培养基中氮源重量的0.1-0.5%，复合蛋白酶活性为20万U/g，通过