ShRNA for targeted knockdown of Atf6 gene expression, recombinant vector and application thereof
The invention provides construction and application of recombinant 8-type adeno-associated virus (rAAV8)-Atf6 shRNA for targeted knockdown of mouse liver Atf6 alpha gene expression, and belongs to the technical field of liver disease treatment research. In the invention, the shRNA targeted nucleotid...
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| creator | LI SUQUN HE YIHUAI WAN DIANWEI XU DELIN LIAO YUE LI YING HUANG MEIYING |
| description | The invention provides construction and application of recombinant 8-type adeno-associated virus (rAAV8)-Atf6 shRNA for targeted knockdown of mouse liver Atf6 alpha gene expression, and belongs to the technical field of liver disease treatment research. In the invention, the shRNA targeted nucleotide sequence is shown as SEQ ID No.1, and the shRNA nucleotide sequence is shown as SEQ ID No.2; The recombinant vector for targeted knockdown of mouse liver Atf6 alpha gene expression comprises a skeleton vector pAAV8 and the shRNA. The shRNA for targeted knockdown of Atf6 gene expression can knock down Atf6 gene expression, can be applied to mouse model research related to intrahepatic endoplasmic reticulum stress, and is beneficial to further understanding of the effect of Atf6 gene expression in liver injury; and therefore, a new thought and a target point are provided for clinically preventing and treating liver diseases.
本发明提供了一种靶向敲减小鼠肝脏Atf6α基因表达的重组8型腺相关病毒(rAAV8)-Atf6α shRNA构建及其应用，属于肝脏疾病治疗研究技术领域，本发明中，所述shRNA靶向的 |
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本发明提供了一种靶向敲减小鼠肝脏Atf6α基因表达的重组8型腺相关病毒(rAAV8)-Atf6α shRNA构建及其应用，属于肝脏疾病治疗研究技术领域，本发明中，所述shRNA靶向的</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CARE OF BIRDS, FISHES, INSECTS ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW BREEDS OF ANIMALS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210420&DB=EPODOC&CC=CN&NR=112680445A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25544,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210420&DB=EPODOC&CC=CN&NR=112680445A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI SUQUN</creatorcontrib><creatorcontrib>HE YIHUAI</creatorcontrib><creatorcontrib>WAN DIANWEI</creatorcontrib><creatorcontrib>XU DELIN</creatorcontrib><creatorcontrib>LIAO YUE</creatorcontrib><creatorcontrib>LI YING</creatorcontrib><creatorcontrib>HUANG MEIYING</creatorcontrib><title>ShRNA for targeted knockdown of Atf6 gene expression, recombinant vector and application thereof</title><description>The invention provides construction and application of recombinant 8-type adeno-associated virus (rAAV8)-Atf6 shRNA for targeted knockdown of mouse liver Atf6 alpha gene expression, and belongs to the technical field of liver disease treatment research. In the invention, the shRNA targeted nucleotide sequence is shown as SEQ ID No.1, and the shRNA nucleotide sequence is shown as SEQ ID No.2; The recombinant vector for targeted knockdown of mouse liver Atf6 alpha gene expression comprises a skeleton vector pAAV8 and the shRNA. The shRNA for targeted knockdown of Atf6 gene expression can knock down Atf6 gene expression, can be applied to mouse model research related to intrahepatic endoplasmic reticulum stress, and is beneficial to further understanding of the effect of Atf6 gene expression in liver injury; and therefore, a new thought and a target point are provided for clinically preventing and treating liver diseases.
本发明提供了一种靶向敲减小鼠肝脏Atf6α基因表达的重组8型腺相关病毒(rAAV8)-Atf6α shRNA构建及其应用，属于肝脏疾病治疗研究技术领域，本发明中，所述shRNA靶向的</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CARE OF BIRDS, FISHES, INSECTS</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW BREEDS OF ANIMALS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDEOgkAQAGksjPqHtddEFIktIRorCrXH9diDC7h7uduoz5fCB1hNMzPT5H7tLlUBVgIohpaUGuhZTN_Im0EsFGpzaIkJ6OMDxeiEVxDIyPPhGFnhRUbHHLkB9H5wBnV0QDsKJHaeTCwOkRY_zpLl6Xgrz2vyUlP0aMa31mWVptv8sMmyfbH7x_kCZpg84A</recordid><startdate>20210420</startdate><enddate>20210420</enddate><creator>LI SUQUN</creator><creator>HE YIHUAI</creator><creator>WAN DIANWEI</creator><creator>XU DELIN</creator><creator>LIAO YUE</creator><creator>LI YING</creator><creator>HUANG MEIYING</creator><scope>EVB</scope></search><sort><creationdate>20210420</creationdate><title>ShRNA for targeted knockdown of Atf6 gene expression, recombinant vector and application thereof</title><author>LI SUQUN ; HE YIHUAI ; WAN DIANWEI ; XU DELIN ; LIAO YUE ; LI YING ; HUANG MEIYING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112680445A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CARE OF BIRDS, FISHES, INSECTS</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW BREEDS OF ANIMALS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LI SUQUN</creatorcontrib><creatorcontrib>HE YIHUAI</creatorcontrib><creatorcontrib>WAN DIANWEI</creatorcontrib><creatorcontrib>XU DELIN</creatorcontrib><creatorcontrib>LIAO YUE</creatorcontrib><creatorcontrib>LI YING</creatorcontrib><creatorcontrib>HUANG MEIYING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LI SUQUN</au><au>HE YIHUAI</au><au>WAN DIANWEI</au><au>XU DELIN</au><au>LIAO YUE</au><au>LI YING</au><au>HUANG MEIYING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>ShRNA for targeted knockdown of Atf6 gene expression, recombinant vector and application thereof</title><date>2021-04-20</date><risdate>2021</risdate><abstract>The invention provides construction and application of recombinant 8-type adeno-associated virus (rAAV8)-Atf6 shRNA for targeted knockdown of mouse liver Atf6 alpha gene expression, and belongs to the technical field of liver disease treatment research. In the invention, the shRNA targeted nucleotide sequence is shown as SEQ ID No.1, and the shRNA nucleotide sequence is shown as SEQ ID No.2; The recombinant vector for targeted knockdown of mouse liver Atf6 alpha gene expression comprises a skeleton vector pAAV8 and the shRNA. The shRNA for targeted knockdown of Atf6 gene expression can knock down Atf6 gene expression, can be applied to mouse model research related to intrahepatic endoplasmic reticulum stress, and is beneficial to further understanding of the effect of Atf6 gene expression in liver injury; and therefore, a new thought and a target point are provided for clinically preventing and treating liver diseases.
本发明提供了一种靶向敲减小鼠肝脏Atf6α基因表达的重组8型腺相关病毒(rAAV8)-Atf6α shRNA构建及其应用，属于肝脏疾病治疗研究技术领域，本发明中，所述shRNA靶向的</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE |
| title | ShRNA for targeted knockdown of Atf6 gene expression, recombinant vector and application thereof |
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