ShRNA for targeted knockdown of Atf6 gene expression, recombinant vector and application thereof

The invention provides construction and application of recombinant 8-type adeno-associated virus (rAAV8)-Atf6 shRNA for targeted knockdown of mouse liver Atf6 alpha gene expression, and belongs to the technical field of liver disease treatment research. In the invention, the shRNA targeted nucleotide sequence is shown as SEQ ID No.1, and the shRNA nucleotide sequence is shown as SEQ ID No.2; The recombinant vector for targeted knockdown of mouse liver Atf6 alpha gene expression comprises a skeleton vector pAAV8 and the shRNA. The shRNA for targeted knockdown of Atf6 gene expression can knock down Atf6 gene expression, can be applied to mouse model research related to intrahepatic endoplasmic reticulum stress, and is beneficial to further understanding of the effect of Atf6 gene expression in liver injury; and therefore, a new thought and a target point are provided for clinically preventing and treating liver diseases. 本发明提供了一种靶向敲减小鼠肝脏Atf6α基因表达的重组8型腺相关病毒(rAAV8)-Atf6α shRNA构建及其应用，属于肝脏疾病治疗研究技术领域，本发明中，所述shRNA靶向的