NON-STANDARD AMINO ACID CONTAINING COMPOSITIONS AND USES THEREOF

Provided herein is a stabilized DNase I polypeptide containing a non-standard amino acid that maintains enzymatic activity even under harsh conditions, such as reducing environments. The stabilized DNase I polypeptide has enzymatic activity in reducing environments that is higher than a corresponding DNase I polypeptide without the non-standard amino acids under the same conditions. Also provided herein are polynucleotides encoding the stabilized DNase I polypeptide, cells for expressing and/or producing the stabilized DNase I polypeptide, and methods of use of the stabilized DNase I polypeptide. 本文中提供了包含非标准氨基酸的稳定化的DNase I多肽，其即使在苛刻条件、例如还原环境下也保持酶活性。所述稳定化的DNase I多肽在还原环境中具有酶活性，所述酶活性高于在相同条件下没有非标准氨基酸的相应DNase I多肽。本文中还提供了编码所述稳定化的DNase I多肽的多核苷酸、用于表达和/或产生所述稳定化的DNase I多肽的细胞，以及使用所述稳定化的DNase I多肽的方法。