Primers and kit for detecting mycoplasma pneumoniae
The invention discloses a loop-mediated isothermal amplification primer group for detecting mycoplasma pneumoniae. The loop-mediated isothermal amplification primer group is composed of the following three pairs of primers: MP2-F3 and MP2-B3, MP2-FIP and MP2-BIP, and MP2-LF and MP2-LP, wherein the n...
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Zusammenfassung: | The invention discloses a loop-mediated isothermal amplification primer group for detecting mycoplasma pneumoniae. The loop-mediated isothermal amplification primer group is composed of the following three pairs of primers: MP2-F3 and MP2-B3, MP2-FIP and MP2-BIP, and MP2-LF and MP2-LP, wherein the nucleotide sequence of the MP2-F3 is shown as SEQ ID No. 1, the nucleotide sequence of the MP2-B3 is shown as SEQ ID No. 2, the nucleotide sequence of the MP2-FIP is shown as SEQ ID No. 3, the nucleotide sequence of the MP2-BIP is shown as SEQ ID No. 4, the nucleotide sequence of the MP2-LF is shown as SEQ ID No. 5, and the nucleotide sequence of the MP2-LP is shown as SEQ ID No. 6. The primer group provided by the invention can significantly improve detection efficiency, shortens detection time to be within 30 minutes, a decrease by a half compared with 60 minutes or more needed in existing loop-mediated isothermal amplification methods, can be used for rapid detection of mycoplasma pneumoniae infection, provides p |
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