Artificial matching type miRNA for controlling gene expression and application thereof

The invention relates to an artificial matching type miRNA for controlling gene expression and application thereof. The present invention provides a novel artificially matched miRNA using a miRNA. The artificial matching type miRNA is single-stranded nucleic acid comprising an X region and a Y region, and is configured as that the 3' terminal of the X region and the 5 ' terminal of the Y region are connected through a linker region with a non-nucleotide structure; the X region comprises a mature miRNA guide chain sequence; and the Y region is a single-stranded nucleic acid comprising a sequence completely complementary to the X region. The artificially matched miRNA makes it possible to inhibit the expression of a target gene. 本发明涉及控制基因表达的人工匹配型miRNA及其用途。本发明提供一种使用miRNA的新的人工匹配型miRNA。所述人工匹配型miRNA为包括X区域和Y区域的单链核酸，并配置如下：X区域的3'末端和Y区域的5'末端经具有非核苷酸结构的连接子区域相连；所述X区域包括成熟miRNA引导链序列；和所述Y区域为包括与所述X区域完全互补的序列的单链核酸。该人工匹配型miRNA使得可抑制靶基因的表达。