CRISPR/Cas9 system suitable for saccharomyces polygene knockout as well as construction method and application of CRISPR/Cas9 system

The invention discloses a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) system suitable for saccharomyces polygene knockout as well as a construction method and application of the CRISPR/Cas9 system. The CRISPR/Cas...

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Bibliographische Detailangaben
Hauptverfasser:	LI HAOHUA, LI SAINI, ZHU MUZI, CEN YOUFEI, YE WEI, ZHANG WEIMIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) system suitable for saccharomyces polygene knockout as well as a construction method and application of the CRISPR/Cas9 system. The CRISPR/Cas9 vector suitable for saccharomyces cerevisiae polygene knockout is constructed, toxin resistance genes pdr5, pdr10 and pdr15 in the CRISPR/Cas9 vector are knocked out, and the antitoxic effect of toxin sensitive saccharomyces cerevisiae is verified through an antitoxic experiment. The invention provides a polygene knockout CRISPR/Cas9 system knockout method of saccharomyces and even other fungi, and provides a reliable model strain for verifying the antitoxic effect of other toxin resistance genes. According to the invention, the terminator is introduced into the homologous repair template in advance to realize gene inactivation, so that the polygene knockout method provided by the invention has the advantages of